SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Ribeiro,S.L.E.; Pereira,H.L.A.; Silva,N.P.; Neves,R.M.S.; Sato,E.I..

The objective of the present research was to evaluate the usefulness of anti-cyclic citrullinated peptide (anti-CCP) antibodies and the IgM rheumatoid factor (IgM RF) test for the differential diagnosis of leprosy with articular involvement and rheumatoid arthritis (RA). Anti-CCP antibodies and IgM RF were measured in the sera of 158 leprosy patients (76 with and 82 without articular involvement), 69 RA patients and 89 healthy controls. Leprosy diagnosis was performed according to Ridley and Jopling classification criteria and clinical and demographic characteristics of leprosy patients were collected by a standard questionnaire. Leprosy patients with any concomitant rheumatic disease were excluded. Serum samples were obtained from all participants and...

Association of leprosy with HLA-DR2 in a Southern Brazilian population

Palavras-chave: Leprosy; Rheumatoid arthritis; Anti-cyclic citrullinated peptide antibodies; Rheumatoid factor.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100010

The association between HLA specificities and leprosy was investigated in a Southern Brazilian population. One hundred and twenty-one patients and 147 controls were typed for HLA-A, B, Cw, DR and DQ. Patients were subdivided into the following subgroups, according to clinical, histological and immunological criteria: lepromatous (N = 55), tuberculoid (N = 32), dimorphous (N = 20), and indeterminate (N = 14). The frequencies of HLA specificities were compared between the total group of patients and controls, and between the same controls and each subgroup of patients. After correction of the probabilities, deviations were not significant, except for the DR2 specificity, which presented a frequency of 44.2% in the total group of patients and 56.3% in the...

Dapsone syndrome with acute renal failure during leprosy treatment: case report

Palavras-chave: HLA; Leprosy; Mycobacterium leprae.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100008

Alves-Rodrigues,Edson Nogueira; Ribeiro,Luciano Correa; Silva,Margareth Dióz; Takiuchi,Arley; Fontes,Cor Jesus Fernandes.

Dapsone syndrome is a rare hypersensitivity reaction to dapsone and is characterized by high fever, papular or exfoliative dermatitis, progressing to liver toxicity and generalized lymphadenopathy, resembling a mononucleosis infection. We report a patient who developed acute renal failure, as well as other complications characteristic of dapsone syndrome, during leprosy treatment. Renal involvement had not been previously described as a dapsone syndrome feature.

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Leprosy; Dapsone syndrome; Acute renal failure.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100014

Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus

BABT

Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow.

In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.

Double-blind trial of the efficacy of pentoxifylline vs thalidomide for the treatment of type II reaction in leprosy

Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007

Sales,A.M.; Matos,H.J.de; Nery,J.A.C.; Duppre,N.C.; Sampaio,E.P.; Sarno,E.N..

Type II reaction in leprosy, or erythema nodosum leprosum (ENL), is often characterized by severe clinical symptoms together with nerve function impairment leading to permanent disabilities. Thalidomide has been shown to be a highly effective drug for the treatment of ENL. It is, however, contraindicated for women of childbearing age due to its teratogenicity. On the other hand, pentoxifylline, used to treat hypercoagulable states, is not teratogenic and, like thalidomide, can inhibit the synthesis of tumor necrosis factor-a and other cytokines. In the present randomized double-blind clinical study we compared the effectiveness of orally administered pentoxifylline vs thalidomide in treating type II reaction in 44 patients. Daily doses of 300 mg...

Expression of the chemokine receptor CXCR4 on lymphocytes of leprosy patients

Palavras-chave: Leprosy; Reactional episodes; Therapy; Pentoxifylline; Thalidomide.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200011

Mendonça,V.A.; Alvim de Melo,G.E.B.; Araújo,M.G.; Borges,V.O.; Costa,R.D.; Martins-Filho,O.A.; Teixeira- Carvalho,A.; Sathler-Avelar,R.; Teixeira,M.M.; Teixeira,A.L..

Leprosy is caused by Mycobacterium leprae, which induces chronic granulomatous infection of the skin and peripheral nerves. The disease ranges from the tuberculoid to the lepromatous forms, depending on the cellular immune response of the host. Chemokines are thought to be involved in the immunopathogenesis of leprosy, but few studies have investigated the expression of chemokine receptors on leukocytes of leprosy patients. In the present study, we evaluated 21 leprosy patients (M/F: 16/5) with a new diagnosis from the Dermatology Outpatient Clinic of the University Hospital, Federal University of Minas Gerais. The control group was composed of 20 healthy members (M/F: 15/5) of the community recruited by means of announcements. The expression of CCR2,...

Genetics and human rights: Two histories: restoring genetic identity after forced disappearance and identity suppression in Argentina and after compulsory isolation for leprosy in Brazil

Palavras-chave: Leprosy; Chemokine receptors; CXCR4; Leukocytes.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200009

Genet. Mol. Biol.

Penchaszadeh,Victor B.; Schuler-Faccini,Lavinia.

Over the past three decades, there has been an accelerated development of genetic technology, leading to its use in human genetic identification for many purposes. Additionally, it has been made explicit that identity is a fundamental human right. A number of historical circumstances have connected these developments. Personal identity is increasingly associated with the preservation and defense of human rights and is a tool to repair the violation of these rights, particularly the right to identity. In this article, we report the use of genetics to support the right to identity in two historical circumstances. First, we report the search, localization, DNA testing and genetic identification of 110 individuals who were appropriated as babies by the...

Knowledge of the patients regarding leprosy and adherence to treatment

Palavras-chave: Genetics and human rights; Suppression of identity; Leprosy; Argentina; Brazil.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200016

Lira,Karlisson Bezerra; Leite,João Jaime Giffoni; Maia,Débora Castelo Branco de Souza Collares; Freitas,Rafael de Mesquita Ferreira; Feijão,Alexsandra Rodrigues.

Leprosy is a chronic infectious disease caused by Mycobacterium leprae. It is known for its great disfiguring capacity and is considered an extremely serious disease to public health worldwide. The state of Ceará ranks 13th in number of cases of leprosy in Brazil, and fourth in Northeastern region, with an average of 2,149 new cases diagnosed every year. This study aimed to evaluate the knowledge of leprosy patients regarding treatment, and to assess the level of treatment adherence and its possible barriers. The study was conducted in the reference center for dermatology, from September 2010 to October 2010, in Fortaleza, Ceará. The study data were collected by means of a structured interview, along with the Morisky-Green test, in order to assess...

Lepromatous leprosy and perianal tuberculosis: a case report and literature review

Palavras-chave: Leprosy; Adherence; Knowledge.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500012

J. Venom. Anim. Toxins incl. Trop. Dis.

Parise-Fortes,Maria Rita; Lastória,Joel Carlos; Marques,Silvio Alencar; Putinatti,Maria Stella Ayres; Stolf,Hamilton Ometto; Marques,Mariângela Ester Alencar; Haddad,Vidal.

Leprosy is a chronic infectious disease caused by Mycobacterium leprae, a microorganism that usually affects skin and nerves. Although it is usually well-controlled by multidrug therapy (MDT), the disease may be aggravated by acute inflammatory reaction episodes that cause permanent tissue damage particularly to peripheral nerves. Tuberculosis is predominantly a disease of the lungs; however, it may spread to other organs and cause an extrapulmonary infection. Both mycobacterial infections are endemic in developing countries including Brazil, and cases of coinfection have been reported in the last decade. Nevertheless, simultaneous occurrence of perianal cutaneous tuberculosis and erythema nodosum leprosum is very rare, even in countries where both...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Mycobacteria; Leprosy; Tuberculosis; Erythema nodosum leprosum.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200506

Leprosy and hepatitis B coinfection in southern Brazil

Leitão,Cleverson; Ueda,Denis; de Moraes Braga,Anna Carolina; Boldt,Angelica B.W.; Messias-Reason,Iara J.T..

To investigate the association of leprosy with hepatitis B virus (HBV) infection, as yet unknown for South Brazil, we assessed hepatitis B virus coinfection in 199 South Brazilian leprosy patients (119 lepromatous, 15 tuberculoid, 30 borderline, 12 undetermined and 23 unspecified) and in 681 matched blood donors by screening for the hepatitis B virus markers HBSAg and anti-HBc, using ELISA. Positive samples were retested and anti-HBc+ only samples were tested for the hepatitis B surface antibody (anti-HBs). There was a strong association between leprosy and hepatitis B virus infection (OR = 9.8, 95% CI = 6.4–14.7; p = 0.004·E−30), as well as an association between HBV infection and lepromatous leprosy, compared to other forms (OR = 2.4, 95% CI = 1.2–4.8; p...

Methemoglobinemia and dapsone levels in patients with leprosy

Palavras-chave: Leprosy; Hepatitis B; Leprosarium; HBV; Mycobacterium leprae.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100008

Vieira,Jose Luiz Fernandes; Riveira,Juan Gonzalo Bardarez; Martins,Ana de Nazaré Silva; Silva,Jorge Pereira da; Salgado,Claudio Guedes.

The objective of this work was to determine the methemoglobinemia and correlate with dapsone levels in multibacillary leprosy patients under leprosy multi-drug therapy. Thirty patients with laboratory and clinical diagnosis of multibacillary leprosy were enrolled. Dapsone was analyzed by high performance liquid chromatography and methemoglobinemia by spectrophotometry. The mean dapsone concentrations in male was 1.42 g/mL and in female was 2.42 g/mL. The mean methemoglobin levels in male was 3.09 µg/mL; 191%, and in female was 2.84 ± 1.67%. No correlations were seen between dapsone levels and methemoglobin in male and female patients. Our results demonstrated that the dosage of dapsone in leprosy treatment does not promote a significant methemoglobinemia.

Nuclear medicine in tropical diseases

Palavras-chave: Methahemoglobinemia; Dapsone; Leprosy.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300022

BABT

Braga,Francisco José Hossri Nogueira.

Tropical diseases affect more people in the world than any other kind of disease, but scintigraphic data on that matter are not so frequent in the literature. Since the geographic regions where such diseases occur are normally very poor, scintillation cameras may not be available. We present a resumed summary of part of what has been done on this subject to-date. Leprosy affects circa 12 million people worldwide and has already been studied by means of the following scintigraphic exams: Gallium-67, 99mTc-MDP or HMDP, 99mTc-colloid or Dextran, 99mTc-DTPA and 99mTc-WBC. Paracoccidiodomycosis is a deep mycosis and such cases may be evaluated by means of Gallium-67, bone scintigraphy, lymphoscintigraphy, hepato-billiary, bone marrow and liver / spleen...

Patterns of intracellular cytokines in CD4 and CD8 T cells from patients with mycobacterial infections

Palavras-chave: Nuclear medicine; Scintigraphy; Tropical diseases; Leprosy; Paracoccidioidomycosis; Blastomycosis; Mucormycosis; Tuberculosis; Neurocysticercosis; Cutaneous and mucous leishmaniasis; Chagas disease.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500002

Antas,P.R.Z.; Sales,J.S.; Pereira,K.C.; Oliveira,E.B.; Cunha,K.S.; Sarno,E.N.; Sampaio,E.P..

Using a short-term bulk culture protocol designed for an intracellular-staining method based on a flow cytometry approach to the frequencies of cytokine-producing cells from tuberculosis and leprosy patients, we found distinct patterns of T cell subset expression. The method also reveals the profile of peak cytokine production and can provide simultaneous information about the phenotype of cytokine-producing cells, providing a reliable assay for monitoring the immunity of these patients. The immune response of Mycobacterium leprae and purified protein derivative (PPD) in vitro to a panel of mycobacteria-infected patients from an endemic area was assessed in primary mononuclear cell cultures. The kinetics and source of the cytokine pattern were measured at...

qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms

Palavras-chave: Tuberculosis; Leprosy; IFN-gamma; TNF-alpha; Anti-hCD28 mAb.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800003

Azevedo,Michelle de Campos Soriani; Ramuno,Natália Mortari; Fachin,Luciana Raquel Vincenzi; Tassa,Mônica; Rosa,Patrícia Sammarco; Belone,Andrea de Faria Fernandes; Diório,Suzana Madeira; Soares,Cleverson Teixeira; Garlet,Gustavo Pompermaier; Trombone,Ana Paula Favaro.

Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy...

Renal involvement in leprosy: retrospective analysis of 461 cases in Brazil

Palavras-chave: Mycobacterium leprae; QPCR; Leprosy; Bacilloscopy.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100071

Silva Júnior,Geraldo Bezerra da; Daher,Elizabeth De Francesco.

BACKGROUND: Renal lesions in leprosy have been extensively described, including amyloidosis, glomerulonephritis, nephrosclerosis, tubulointerstitial nephritis and granulomas. MATERIAL AND METHODS: A retrospective study was designed to detect renal function abnormalities in 461 leprosy patients, without any co-morbidity, seen in a university hospital in northeast Brazil. The laboratory test results concerning renal function were examined in the patients' medical records. RESULTS: The mean age was 39 ± 18 years and 217 (47%) were male. The mean duration of disease was 21 ± 38 months. Levels of creatinine above 1.4mg/dL had been detected in 40 patients (8.6%). The levels of creatinine and urea were higher in lepromatous leprosy patients. Proteinuria,...

Salivary anti-PGL IgM and IgA titers and serum antibody IgG titers and avidities in leprosy patients and their correlation with time of infection and antigen exposure

Palavras-chave: Leprosy; Kidney; Renal abnormalities; Renal failure.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200007

Nagao-Dias,Aparecida T.; Almeida,Thereza Lúcia P.; Oliveira,Maria de Fátima; Santos,Rivanda C.; Lima,Ana Laura P.; Brasil,Márcia.

The present work proposed to correlate serum antibody avidity and salivary antibody titers as parameters for time of infection and antigen exposure in a co-hort study evaluating leprosy patients in different periods of treatment. Colorimetric enzyme-immunoassays for salivary antibodies, serum antibody IgG titers and avidities were performed in the samples. Anti-PGL-1 IgA and IgM salivary antibodies were significantly higher in multibacillar (MB-L) patients compared to normal controls (p<0.05), but not when compared to borderline tuberculoid (BT) or to paucibacillar (PB-L) patients (p>0.05). A good correlation was found between salivary anti-PGL-1 IgA and IgM levels in MB-L patients (r=0.41, p<0.01). Two out of 33 tested saliva samples from...

Salivary anti-PGL-1 IgM may indicate active transmission of Mycobacterium leprae among young people under 16 years of age

Palavras-chave: Anti-PGL-1; Avidity; Leprosy; Salivary antibodies.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200009

Macedo,Alexandre Casimiro de; Cunha Jr.,José Evandro; Yaochite,Juliana Navarro Ueda; Tavares,Clodis Maria; Nagao-Dias,Aparecida Tiemi.

Abstract Considering that the main route of Mycobacterium leprae transmission is the upper respiratory tract, detection of salivary antibodies can be a useful tool for diagnosing early infection. The study aimed to analyze salivary anti-PGL-1 IgA and IgM antibodies in 169 children aged 4-16 years old, who lived nearby or inside the house of multibacillary or paucibacillary leprosy patients in two endemic cities in Alagoas State - Brazil. Salivary anti-PGL-1 antibodies were quantified by modified ELISA method. The frequency of contact and clinical form of the index case were significantly associated with salivary antibody levels. High frequency of IgM positivity strongly suggests active transmission of M. leprae in these communities. We suggest in the...

The role of ERBB2 gene polymorphisms in leprosy susceptibility

Palavras-chave: Leprosy; Salivary antibodies; Phenolic glycolipid-1 antigen; Mycobacterium leprae.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500557

Rêgo,Jamile Leão; Oliveira,Joyce Moura; Santana,Nadja de Lima; Machado,Paulo Roberto Lima; Castellucci,Léa Cristina.

Mycobacterium leprae infects skin and peripheral nerves causing deformities and disability. The M. leprae bacterium binds to ErbB2 on the Schwann cell surface causing demyelination and favoring spread of the bacilli and causing nerve injury. Polymorphisms at the ERBB2 gene were previously investigated as genetic risk factors for leprosy in two Brazilian populations but with inconsistent results. Herein we extend the analysis of ERBB2 variants to a third geographically distinct population in Brazil. Our results show that there is no association between the genotyped SNPs and the disease (p > 0.05) in this population. A gene set or pathway analysis under the genomic region of ERBB2 will be necessary to clarify its regulation under M. leprae stimulus.

Use of anti-PGL-1 antibodies to monitor therapy regimes in leprosy patients

Palavras-chave: ERBB2; Gene analysis; Leprosy.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200206

Zenha,E.M.R.; Ferreira,M.A.N.; Foss,N.T..

The suitability of IgM antibodies to PGL-1 for monitoring the response to multidrug therapy (MDT) was sequentially tested by ELISA in 105 leprosy patients, and bacterial indexes (BI) were also determined. Patients were divided into 3 groups: group 1, 34 multibacillary (MB) patients treated for 12 months with MDT-MB; group 2, 33 MB patients treated for 24 months with MDT-MB, and group 3, 38 paucibacillary (PB) patients treated for 6 months with MDT-PB. Untreated MB patients exhibited higher antibody levels (mean ± SEM): group 1 (6.95 ± 1.35) and group 2 (12.53 ± 2.02) than untreated PB patients (1.28 ± 0.35). There was a significant difference (P < 0.01) in anti-PGL-1 levels in group 1 patients: untreated (6.95 ± 1.35) and treated for 12 months (2.78 ±...

Use of the polymerase chain reaction to detect Mycobacterium leprae in urine

Palavras-chave: Leprosy; Anti-PGL-1; Multidrug therapy; MDT 12 months; MDT 24 months.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000016

Caleffi,K.R.; Hirata,R.D.C.; Hirata,M.H.; Caleffi,E.R.; Siqueira,V.L.D.; Cardoso,R.F..

Leprosy is an infectious disease caused by Mycobacterium leprae. The polymerase chain reaction (PCR) has been applied to detect M. leprae in different clinical samples and urine seems to be attractive for this purpose. PCR was used to improve the sensitivity for diagnosing leprosy by amplifying a 151-bp PCR fragment of the M. leprae pra gene (PCR-Pra) in urine samples. Seventy-three leprosy patients (39 males and 34 females, 14 to 78 years old) were selected for leprosy diagnosis at a reference laboratory in Maringá, PR, Brazil. Of these, 36 were under anti-leprosy multidrug therapy with dapsone and rifampicin for tuberculoid (TT) and dapsone, rifampicin and clofazimine for borderline (BB) and lepromatous (LL) forms. The control group contained 50 healthy...